有效的抗支原體抗生素

Autoclaving the contaminated cell cultures is the best way to get rid of the infections. In the case of valuable cells contaminated by mycoplasmas, autoclave cannot be helpful and an elimination method should be used without harming the eukaryotic cells. Besides the treatment of cell cultures, surfaces, cell culture media and supplements can also be treated by different methods including autoclaving, filtration, exposure to detergents, culture in the presence of 6-methylpurine deoxyriboside, passage through nude mice, and antibiotic treatment (77).

To eradicate mycoplasmas from FBS and trypsin, UV irradiation is more effective than the mentioned methods. In contrast to gamma irradiation, UV irradiation does not harm the serum components. UV irradiation can eliminate any potential contamination in trypsin or FBS (78).

A frequent check up is needed to verify a complete eradication of mycoplasmas. Sometimes mycoplasmas are suppressed and their titer decreases below detection levels of available assays. Thus, a sensitive mycoplasma testing is necessary to detect any reinfection in cell cultures. It is advised to recheck the cells in antibiotic-free medium after at least four to six subculture passages.

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